Protective Effect of Quercetin Administration in Bacterial- induced Periodontitis on Rats
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Abstract
Introduction: Quercetin is a flavonoid found in a variety of plants, including guava, apples, onions, and tea. It’s been used as an anti-oxidant and anti-inflammatory substance for a long time. This study aims to investigate the effect of quercetin on periodontitis caused by Porphyromonas gingivalis-adhered ligatures. Methods: Eighteen male adult Sprague Dawley rats were divided into 3 groups, namely the control group (C, n=6) and the other two groups that received quercetin at 45mg/kg/day as a preventive (Qp, n=6) and a curative treatment (Qc, n=6), respectively. Under general anaesthesia, periodontitis was induced by placing a 3/0 non-resorbable sterile silk thread around the mandibular incisor teeth of eighteen male adult Sprague Dawley rats. The ligature placement caused severe irritation in the periodontal tissue. The animals were euthanized after 14 days of post-induction treatment, and samples of the mandibular portion were kept in formalin and prepared for histological processing to determine the grade of inflam- mation (GI). The periodontal pocket depth (PPD) was measured using the Michigan-O probe with Williams marks at the mesial and lingual sites of the rat’s incisors tooth to determine the clinical parameter. Results: Qp showed the best improvement, in both parameters, clinically (PPD score, p=0,0018 at the lingual site, and p=0,0264 at the me- sial site) and histologically (GI, p=0,0002). Significant differences were found in preventing clinical attachment-loss statistically (p<0,05) on Qp, better than the Qc at an equal dose (p<0,05). Conclusion: This finding suggests that quercetin administered as a preventive measure (Qp) may promote the healing process of gingiva in periodontitis conditions better than the control group and curative group (Qc).
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Genco RJ. Current view of risk factors for periodontal diseases. Journal of periodontology. 1996 Oct;67:1041-9. doi: 10.1902/jop.1996.67.10.1041
Pai PG, Dayakar MM, Nath AR, Ashwini G. Phytotherapeutics in the management of periodontal disease-A review. SRM Journal of Research in Dental Sciences. 2019 Apr 1;10(2):82.. doi: 10.4103/srmjrds.srmjrds_41_18
Xu D, Hu MJ, Wang YQ, Cui YL. Antioxidant activities of quercetin and its complexes for medicinal application. Molecules. 2019 Jan;24(6):1123. doi: 10.3390/molecules24061123
Caddeo C, Gabriele M, Fernàndez-Busquets X, Valenti D, Fadda AM, Pucci L, Manconi M. Antioxidant activity of quercetin in Eudragit-coated liposomes for intestinal delivery. International journal of pharmaceutics. 2019 Jun 30;565:64-9. doi: 10.1016/j.ijpharm.2019.05.007
Hapner CD, Deuster P, Chen Y. Inhibition of oxidative hemolysis by quercetin, but not other antioxidants. Chemico-biological interactions. 2010 Aug 5;186(3):275-9. doi: 10.1016/j. cbi.2010.05.010
Gatto MT, Falcocchio S, Grippa E, Mazzanti G, Battinelli L, Nicolosi G, Lambusta D, Saso L. Antimicrobial and anti-lipase activity of quercetin and its C2-C16 3-O-acyl-esters. Bioorganic & medicinal chemistry. 2002 Feb 1;10(2):269-72. doi: 10.1016/s0968-0896(01)00275-9
Geoghegan F, Wong RW, Rabie AB. Inhibitory effect of quercetin on periodontal pathogens in vitro. Phytotherapy Research. 2010 Jun;24(6):817-20. doi: 10.1002/ptr.3014
Gupta GK, Chahal J, Arora D. Psidium guajava Linn.: Current research and future prospects. J Pharm Res. 2011 Jan 4;4(1):42-6.
Mohammadi-Sartang M, Mazloom Z, Sherafatmanesh S, Ghorbani M, Firoozi D. Effects of supplementation with quercetin on plasma C-reactive protein concentrations: a systematic review and meta-analysis of randomized controlled trials. European journal of clinical nutrition. 2017 Sep;71(9):1033-9. doi: 10.1038/ejcn.2017.55
Talmac AC, Calisir M, Eroglu EG, Ertugrul AS. Effects of Er, Cr: YSGG and diode lasers on clinical parameters and gingival crevicular fluid IL-1β and IL-37 levels in generalized aggressive periodontitis. Mediators of inflammation. 2019 Jun 12;2019. doi: 10.1155/2019/2780794
Xiong G, Ji W, Wang F, Zhang F, Xue P, Cheng M, Sun Y, Wang X, Zhang T. Quercetin inhibits inflammatory response induced by LPS from Porphyromonas gingivalis in human gingival fibroblasts via suppressing NF-κB signaling pathway. BioMed research international. 2019 Aug 20;2019. doi: 10.1155/2019/6282635
Wei Y, Fu J, Wu W, Ma P, Ren L, Yi Z, Wu J.
Quercetin Prevents Oxidative Stress-Induced Injury of Periodontal Ligament Cells and Alveolar Bone Loss in Periodontitis. Drug Design, Development and Therapy. 2021;15:3509. doi: 10.2147/DDDT. S315249
Falcao A, Bullón P. A review of the influence of periodontal treatment in systemic diseases. Periodontology 2000. 2019 Feb;79(1):117-28. doi: 10.1111/prd.12249
Wikesjö UM, Nilvéus RE, Selvig KA. Significance of early healing events on periodontal repair: a review. Journal of periodontology. 1992 Mar;63(3):158-65. doi: 10.1902/jop.1992.63.3.158
Xiong G, Ji W, Wang F, Zhang F, Xue P, Cheng M, Sun Y, Wang X, Zhang T. Quercetin inhibits inflammatory response induced by LPS from Porphyromonas gingivalis in human gingival fibroblasts via suppressing NF-κB signaling pathway. BioMed research international. 2019 Aug 20;2019. doi: 10.1155/2019/6282635
Aithal GC, Nayak UY, Mehta C, Narayan R, Gopalkrishna P, Pandiyan S, Garg S. Localized in situ nanoemulgel drug delivery system of quercetin for periodontitis: Development and computational simulations. Molecules. 2018 Jun;23(6):1363. doi: 10.3390/molecules23061363
De David SC, Mário TG, De Freitas GC, Kantorski KZ, Wikesjö UM, Moreira CH. Correlation between plaque control and gingival health using short and extended oral hygiene intervals. Clinical oral investigations. 2018 Sep;22(7):2593-7. doi: 10.1007/s00784-018-2358-5
Demkovych AY, Bondarenko YI, Stakhanska OO. The Effects of Quercetin to Glutathione System in the Bacterial-Immune Periodontitis Development. Український журнал медицини, біології та спорту. 2018(3,№ 4):20-4. doi: 10.26693/jmbs03.04.020
Taskan MM, Gevrek F. Quercetin Decreased Alveolar Bone Loss and Apoptosis in Experimentally Induced Periodontitis Model in Wistar Rats. Anti-inflammatory & Anti-Allergy Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry- Anti-Inflammatory and Anti-Allergy Agents). 2020 Dec 1;19(4):436-48. doi: 10.2174/187152301966 6200124114503
Bainbridge P. Wound healing and the role of fibroblasts. Journal of wound care. 2013 Aug 1;22(8). doi: 10.12968/jowc.2013.22.8.407
Cheng WC, Huang RY, Chiang CY, Chen JK, Liu CH, Chu CL, Fu E. Ameliorative effect of quercetin on the destruction caused by experimental periodontitis in rats. Journal of periodontal research. 2010 Dec;45(6):788-95. doi: 10.1111/j.1600-
2010.01301.x
Beken B, Serttas R, Yazicioglu M, Turkekul K, Erdogan S. Quercetin Improves Inflammation, Oxidative Stress, and Impaired Wound Healing in Atopic Dermatitis Model of Human Keratinocytes. Pediatric Allergy, Immunology, and Pulmonology. 2020 Jun 1;33(2):69-79. doi: 10.1089/ ped.2019.1137
Chan CY, Lien CH, Lee MF, Huang CY. Quercetin suppresses cellular migration and invasion in human head and neck squamous cell carcinoma (HNSCC). Biomedicine. 2016 Sep;6(3). doi: 10.7603/s40681-016-0015-3