Assessment of Selected Antioxidants Biomarkers Activity of Cissampelos owariensis Fractions in Streptozotocin-induced Diabetic Wistar Rats
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Abstract
Introduction: The market for herbal medicines with anti-diabetic qualities is still quite considerable on a global scale. The current study examines the actions of a few biomarkers in adult male Wistar rats fed with Cissampelos owariensis fractionated leaf extract in methanol. Materials and methods: Fresh C. owariensis leaf was stitched, dried, and fractionated using chloroform, n-hexane, and ethyl acetate solvents after being air-dried, crushed, and submerged in methanol for 72 hours. Thirty (30) mature male Wistar rats weighing 130–160 grams were divided into six (6) groups of five rats (n=5) each. Streptozotocin (STZ) (60 mg/kg) was injected intraperitoneally once to cause diabetes. Groups 1 and 2 serve as normal and diabetic control rats, respectively, while Group 3 diabetic rats received 50 mg/kg/day of metformin and Groups 4, 5, and 6 received 250 mg/kg/day of C. owariensis fractions for 21 days before being euthanized to harvest the kidney, liver, small intestine, brain, and pancreas for biochemical study. Groups 1 and 2 are used as the diabetic and normal control groups of rats, respectively. The generated data was examined with the aid of the SPSS program, and the results were presented as mean SEM. Results: The results of the investigation showed that diabetic rats' increased blood glucose levels were significantly lower than control groups, indicating that the ethyl acetate fraction had the strongest hypoglycemic potential of the fractions. Alpha-amylase, alpha-glucosidase, and malondialdehyde (MDA) were significantly inhibited by C. owariensis fractions whereas Superoxide dismutase (SOD) and Catalase (CAT) activity were significantly boosted in diabetic rats. Conclusion: In conclusion, C. owariensis fractions showed strong anti-diabetic and pancreatic-protecting activities in animal models.
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