X-chromosome Short Tandem Repeats Resolved Complex Kinship Cases in Malaysia: Case Reports

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Published: Sep 30, 2025
DOI: https://doi.org/10.47836/mjmhs.21.5.52
Keywords:
X-chromosome, Short tandem repeats, Kinship, Forensic, Malaysia

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Aedrianee Reeza Alwi
(1) Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia. (2) Department of Chemistry Malaysia Johor State, Jalan Abdul Samad, 80100 Johor Bahru, Johor, Malaysia.
Naji Arafat Mahat
(1) Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia. (2) Investigative and Forensic Sciences Research Group, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia. (3) Centre for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia. (4) Centre of Research for Fiqh Forensics and Judiciary, Faculty of Syariah and Law, Universiti Sains Islam Malaysia (USIM), Bandar Baru Nilai, 71800, Nilai, Negeri Sembilan, Malaysia.
Nor Arina Syafawati Abd Aziz
Department of Chemistry Malaysia Johor State, Jalan Abdul Samad, 80100 Johor Bahru, Johor, Malaysia.
Nur Hafiza Md Yusop
Department of Chemistry Malaysia Johor State, Jalan Abdul Samad, 80100 Johor Bahru, Johor, Malaysia.
Wan Nur Zawani Wan Mohd Samsuddin
Department of Chemistry Malaysia Johor State, Jalan Abdul Samad, 80100 Johor Bahru, Johor, Malaysia.
Faezah Mohd Salleh
(1) Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia. (2) Investigative and Forensic Sciences Research Group, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia.
Seri Mirianti Ishar
Forensic Science Program, Faculty of Health Sciences, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, Malaysia.
Mohammad Rahim Kamaluddin
Psychology and Human Well Being Research Centre, Faculty of Social Sciences and Humanities, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, Malaysia.
Mohd Radzniwan A. Rashid
Family Medicine Unit, Faculty of Medicine and Health Science, Universiti Sains Islam Malaysia (USIM), Bandar Baru Nilai, 71800, Nilai, Negeri Sembilan, Malaysia.
Sharifah Nany Rahayu Karmilla Syed Hassan
Faculty of Medicine, Universiti Sultan Zainal Abidin (UniSZA), Medical Campus, 20400 Kuala Terengganu, Terengganu, Malaysia.

Abstract

The article reports the first use of X-chromosome short tandem repeats (X-STR) in two Malaysian cases viz. disputed siblings and deficiency maternity (maternal grandparents). X-STR profiling from blood on FTA cards using 12 loci successfully proved half-siblings relationships and established the maternal grandmother-child relationship, highlighting the potential of X-STR analysis in resolving kinship investigations for forensic casework in Malaysia.

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How to Cite
Alwi, A. R., Naji Arafat Mahat, Abd Aziz, N. A. S., Md Yusop, N. H., Wan Mohd Samsuddin, W. N. Z., Mohd Salleh, F., Ishar, S. M., Mohammad Rahim Kamaluddin, A. Rashid, M. R., & Syed Hassan, S. N. R. K. (2025). X-chromosome Short Tandem Repeats Resolved Complex Kinship Cases in Malaysia: Case Reports. Malaysian Journal of Medicine and Health Sciences, 21(5), 441–444. https://doi.org/10.47836/mjmhs.21.5.52
Issue
Vol. 21 No. 5 (2025)
Section
Case Report
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References

Periera V, Gusmão L. The X-Chromosomal STRs in Forensic Genetics: X Chromosome STRs. In: Pilli E, Berti A, editors. Forensic DNA analysis technologies development and innovative applications. Canada: CRC Press; 2021, p. 91–112.

Gomes C, Arroyo-Pardo E. Usefulness of the X-Chromosome on forensic science. In: Dash HR, Shirvastava P, Lorente JA, editors. Handbook of DNA Profiling. Singapore: Springer; 2022, p. 455–77. https://doi.org/10.1007/978-981-16-4318-7_17.

Alwi AR, Mahat NA, Mohd Salleh F, Ishar SM, Kamaluddin MR, Rashid MRA. Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards. J Forensic Sci 2023; 68 (6): 2103-2115 https://doi.org/10.1111/1556-4029.15370.

Scientific Working Group on DNA Analysis Methods (SWGDAM). Recommendations of the SWGDAM Ad Hoc Working Group on genotyping results reported as likelihood ratios. 2018.https://www.swgdam.org/_files/ugd/4344b0_dd5221694d1448588dcd0937738c9e46.pdf. (accessed May 2, 2024).

Nunzio C Di, Nunzio M Di, Saia F, Ricci P, Nunzio A Di, Presciuttini S. X-chromosome analysis in an unusual deficiency maternity case. Forensic Sci Int Genet Suppl Ser 2019; 7:p.365–367. https://doi.org/10.1016/j.fsigss.2019.10.014.