Detection of Pathogenic Leptospira in Rats and Phylogenetic Analysis Using Outer Membrane Lipoprotein Lipl32 Gene at Two Major Public Markets
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Abstract
Introduction: Limited studies have been documented on the presence of pathogenic Leptospira in public markets serving the community in sub-districts of Selangor. The aim of this study was to detect the presence of pathogenic Leptospira in rats using a gene encoding an outer membrane lipoprotein LipL32. Methods: Polymerase chain reaction (PCR) was performed using LipL32 primers on sixty kidney samples of rats trapped at two locations of study; Pasar Borong Selangor in Seri Kembangan and Pasar Basah Bandar Baru Bangi in Bangi. Results: Out of 60 samples analysed, 36.7% were positive for the presence of LipL32. All positive samples highly matched (>94%) nucleotide sequence for LipL32 of pathogenic Leptospira and related to the pathogens through phylogenetic analysis. Conclusion: The detection of LipL32 indicates the potential presence of pathogenic Leptospira species at public markets. Although only 60 rats were successfully trapped, the rats are mobile and might further transmit the pathogenic organisms to other areas.
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Picardeau M. Virulence of the zoonotic agent of leptospirosis: still terra incognita? Nat Rev Microbiol. 2017;15(5):297-307.
Barragan V, Nieto N, Keim P, Pearson T. Meta-analysis to estimate the load of Leptospira excreted in urine: beyond rats as important sources of transmission in low-income rural communities. BMC Res Notes. 2017;10(1):71.
Haake DA, Levett PN. Leptospirosis in humans. Curr Top Microbiol Immunol. 2015;387:65-97.
Ahmed N, Devi SM, Valverde Mde L, Vijayachari P, Machang’u RS, Ellis WA, et al. Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species. Ann Clin Microbiol Antimicrob. 2006;5:28.
Adler B, Lo M, Seemann T, Murray GL. Pathogenesis of leptospirosis: the influence of genomics. Vet Microbiol. 2011;153(1-2):73-81.
Hoke DE, Egan S, Cullen PA, Adler B. LipL32 is an extracellular matrix-interacting protein of Leptospira spp. and Pseudoalteromonas tunicata. Infect Immun. 2008;76(5):2063-9.
Vieira ML, Atzingen MV, Oliveira TR, Oliveira R, Andrade DM, Vasconcellos SA, et al. In vitro identification of novel plasminogen-binding receptors of the pathogen Leptospira interrogans. PLoS One. 2010;5(6):e11259.
Ridzlan FR, Bahaman AR, Khairani-Bejo S, Mutalib AR. Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia. Trop Biomed. 2010;27(3):632-8.
Samsudin S, Masri SN, Jamaluddin TZMT, Siti Nor Sakinah Saudi SN, Ariffin UMK, Amran F, et al. Seroprevalence of Leptospiral antibodies among healthy municipal service workers in Selangor. Hindawi Publishing Corporation. 2015;2015:6.
Faulde M, Freise J. [Public health pests. Arthropods and rodents as causative disease agents as well as reservoirs and vectors of pathogens]. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2014;57(5):495-503.
Bahaman AR, Ibrahim AL. A review of leptospirosis in Malaysia. Vet Res Commun. 1988;12(2-3):179-89.
Benacer D, Mohd Zain SN, Amran F, Galloway RL, Thong KL. Isolation and molecular characterization of Leptospira interrogans and Leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia. Am J Trop Med Hyg. 2013;88(4):704-9.
Hasoun GA, Bahaman AR, Bejo SK, Zakaria Z, Garba B. Serological and molecular prevalence of Leptospira infection in rat populations in Kuala Lumpur. Aust J Basic & Appl Sci. 2017;11(1):62-72.
Mohamed-Hassan SN, Bahaman AR, Mutalib SKB. Prevalence of pathogenic leptospires in rats from selected locations in Peninsular Malaysia. Research Journal of Animal Sciences. 2012;6(1):12-25.
Dzulaikha K NYM, Maizatulriah JJ, Marfiah AW editor Association of rainfall and the occurrence of pathogenic Leptospira spp. in recreational stream water, Hulu Langat, Selangor. 2nd International Conference for Innovation in Biomedical Engineering and Life Sciences: ICIBEL 2017 (in conjunction with APCMBE 2017),10 - 13 December 2017; 2018; Penang Malaysia: Springer Singapore.
Khairani-Bejo S, Oii SS, Bahaman AR. Rats: Leptospirosis reservoir in Serdang Selangor residential area. Journal of Animal and Veterinary Advances. 2004;3(2):66-9.
Garba B, Bahaman AR, Khairani-Bejo S, Zakaria Z, Mutalib AR. Retrospective Study of Leptospirosis in Malaysia. Ecohealth. 2017;14(2):389-98.
Malmstrom J, Beck M, Schmidt A, Lange V, Deutsch EW, Aebersold R. Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans. Nature. 2009;460(7256):762-5.
Kumaran SK, Bakar MFA, Mohd-Padil H, Mat-Sharani S, Sakinah S, Poorani K, et al. 3D modelling of the pathogenic Leptospira protein LipL32: A bioinformatics approach. Acta Trop. 2017;176:433-9.
Lin X, Zhao J, Qian J, Mao Y, Pan J, Li L, et al. Identification of immunodominant B- and T-cell combined epitopes in outer membrane lipoproteins LipL32 and LipL21 of Leptospira interrogans. Clin Vaccine Immunol. 2010;17(5):778-83.
Murray GL. The lipoprotein LipL32, an enigma of Leptospiral biology. Vet Microbiol. 2013;162(2-4):305-14.
Ellis WA. Animal leptospirosis. Curr Top Microbiol Immunol. 2015;387:99-137.
Kamath N, Iyengar A. Infections and the kidney: a tale from the tropics. Pediatr Nephrol. 2018;33(8):1317-26.
Haake DA, Suchard MA, Kelley MM, Dundoo M, Alt DP, Zuerner RL. Molecular evolution and mosaicism of Leptospiral outer membrane proteins involves horizontal DNA transfer. J Bacteriol. 2004;186(9):2818-28.
Foronda P, Martin-Alonso A, Del Castillo-Figueruelo B, Feliu C, Gil H, Valladares B. Pathogenic Leptospira spp. in wild rodents, Canary Islands, Spain. Emerg Infect Dis. 2011;17(9):1781-2.
Pui CF, Bilung LM, Apun K, Su’ut L. Diversity of Leptospira spp. in Rats and Environment from Urban Areas of Sarawak, Malaysia. J Trop Med. 2017;2017:3760674.
Mohamad-Ikbal NH, Bhassu S, Simarani K, Uni S, Chan CC, Omar H. Prevalence and genetic divergence of Leptospira interrogans and Leptospira borgpetersenii in house rats (Rattus rattus) from Peninsular Malaysia. Asian Pac J Trop Med. 2019;12(10):463-71.