High Prevalence of Methicillin-resistant Staphylococcus haemolyticus Isolated From Commensals in Healthy Adults

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Published: Jan 31, 2021
Keywords:
Commensals, mecA, MRSH, MSSH, SCCmec

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Farhan Haziq Azharollah
School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450, Shah Alam, Selangor, Malaysia
Mohd Faiz Foong Abdullah
School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450, Shah Alam, Selangor, Malaysia
Siti Farah Alwani Mohd Nawi
Clinical Training Centre, Faculty of Medicine, Universiti Teknologi MARA, 47000, Sungai Buloh, Selangor, Malaysia
Aziyah Abdul-Aziz
School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450, Shah Alam, Selangor, Malaysia

Abstract

Introduction: S. haemolyticus is known to be commensals residing on human skin. However, their ability to develop as pathogens among the healthy community has becoming increasingly vital. Methods: In this study, a total of 49 non-duplicated samples of S. haemolyticus was isolated from the skin of healthy adults and confirmed via sodA gene sequencing method. Cefoxitin (30μg) disc diffusion test was performed to determine methicillin resistance among the S. haemolyticus isolates. The isolates were then subjected to mecA amplification and Staphylococcus Cassette Chromosome (SCCmec) typing of I, II, III, IV and V. Results: Interestingly, 59.2% of the S. haemolyticus commensal isolates were found to be methicillin-resistant (MRSH) while the remaining 40.8% was methicillin-sensitive (MSSH). Amplification of mecA gene showed that 43 isolates (87.8%) were positive while only six isolates were negative for the gene. A majority of the positive mecA isolates (90.7%) were discovered to harbour SCCmec Type II while the re- maining 44.2% were Type V followed by 23.3% of Type I and 18.6% of Type IV. Only one of the isolates was found to be SCCmec Type III while another isolate, T187 was non-typeable. Conclusion: The data indicates the acquisition of SCCmec typing circulated among the commensal strains which could be a potential route of pathogenicity among the isolates.

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Azharollah, F. H., Foong Abdullah, M. F., Mohd Nawi, S. F. A., & Abdul-Aziz, A. (2021). High Prevalence of Methicillin-resistant Staphylococcus haemolyticus Isolated From Commensals in Healthy Adults. Malaysian Journal of Medicine and Health Sciences, 17(1), 145–152. Retrieved from http://mjmhsojs.upm.edu.my/index.php/mjmhs/article/view/175
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Vol. 17 No. 1 (2021)
Section
Original Articles

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