Effect of Systemic Administration of Granulocyte-Colony Stimulating Factor on Rate of Fracture Healing of Bone Defect in Goats as Animal Model
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Abstract
Granulocyte-colony stimulating factor (G-CSF) serves as an important cytokine in haematopoiesis; released at both
physiological and pathological conditions by a range of cells. We hypothesized that the systemic administration of
G-CSF would produce an accelerated fracture-healing rate in non-union bone defects; thus, potentially leading to
useful clinical applications. Ten male adult Katjang goats, weighing about 15-26 kilograms were randomly chosen
and a tibial bone defect was induced in each animal. The defect was maintained by internal fixation with a titanium
plate and reinforced by an external fiberglass cast. Post-operative radiographs were performed twice weekly and
radiographic assessments were performed by evaluating the bridging and union measurements through a validated
method. In the treatment group, the time for bridging and union exhibited statistically significant differences when
compared with a control group. The outcomes of the present study establishing a notion that administration of G-CSF
besides inducing haematopoiesis, promotes healing of fractures and non-union bone defects as well.
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