Polymeric Material With Controlled Release of Antimicrobial Agents for Medical Application

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Published: May 31, 2022
Keywords:
Wound infection, Poly(2-hydroxyethyl methacrylate), Controlled release, Local antimicrobial agents, Bandages

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Vasyl Nagaichuk
Department of General Surgery, National Pirogov Memorial Medical University, Pirogov str. 56, Vinnytsya, 21028, Ukraine.
Igor Gerashchenko
Department of Biomedical Problems surface No6, Chuiko Institute of Surface Chemistry, NAS of Ukraine, General Naumov str. 17, Kyiv, 03164, Ukraine.
Roman Chornopyshchuk
Department of General Surgery, National Pirogov Memorial Medical University, Pirogov str. 56, Vinnytsya, 21028, Ukraine.
Oleksandr Nazarchuk
Department of Microbiology, National Pirogov Memorial Medical University, Pirogov str. 56, Vinnytsya, 21028, Ukraine.
Olena Kukolevska
Department of Pharmaceutical chemistry, National Pirogov Memorial Medical University, Pirogov str. 56, Vinnytsya, 21028, Ukraine.
Mariia Faustova
Department of microbiology, virology and immunology, Poltava State Medical University, Shevchenko str. 23, Poltava, 36011, Ukraine.
Mykola Burkovskyi
Department of General Surgery, National Pirogov Memorial Medical University, Pirogov str. 56, Vinnytsya, 21028, Ukraine.

Abstract

Introduction: Nowadays the use of synthetic polymers has become an integral part of modern medicine. Poly(2-hy- droxyethyl methacrylate) has attracted special attention for therapeutic use. The objective of this study was to devel- op novel polymeric material based on poly(2-hydroxyethyl methacrylate) by addition of water as pore-forming agent and antimicrobial components, which would differ from similar materials by controlled release of active substances. Methods: The antimicrobial release kinetics study materials were immersed into distilled water followed by sampling and measuring their concentration. Concentration of chlorhexidine bigluconate and metronidazole was determined using spectrophotometric method and decamethoxine by photocolorimetric method based on reaction with eosin. The swelling rate was determined by gravimetric method. Results: Conventional dressing materials, after being soaked with antiseptic solutions, have demonstrated limited abilities in releasing active substances. Gauze pads were found to release antimicrobials during a short period of time reaching 50–80 % for decamethoxine containing samples and almost 100 % for those with metronidazole and chlorhexidine bigluconate at 2 h of observation. No study active substances were released from activated charcoal dressings. Similar results were obtained with porcine xenografts. Unlike the above mentioned dressing materials, modified polymer matrix based on poly(2-hydroxyethyl methacrylate) showed the controlled release of antimicrobial substances into water medium. Study material contain- ing 3.0 % of decamethoxine and 76.3 % of water demonstrated optimal efficiency in the rate and duration of release, exerting high physical and mechanical properties. Conclusion: The synthesized polymers are similar to conventional dressings in antimicrobial release kinetics, but in some characteristics they are better for practical application.

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How to Cite
Nagaichuk, V., Gerashchenko, I., Chornopyshchuk, R., Nazarchuk, O., Kukolevska, O., Faustova, M., & Burkovskyi, M. (2022). Polymeric Material With Controlled Release of Antimicrobial Agents for Medical Application. Malaysian Journal of Medicine and Health Sciences, 18(3), 11–16. Retrieved from http://mjmhsojs.upm.edu.my/index.php/mjmhs/article/view/60
Issue
Vol. 18 No. 3 (2022)
Section
Original Articles

References

Liu S, Zheng Y, Liu R, Tian C. Preparation and characterization of a novel polylactic acid/ hydroxyapatite composite scaffold with biomimetic micro-nanofibrous porous structure. J Mater Sci Mater Med. 2020 Aug 3;31(8):74. https://doi.org/10.1007/ s10856-020-06415-4.

Boateng J, Catanzano O. Advanced Therapeutic Dressings for Effective Wound Healing--A Review. J Pharm Sci. 2015 Nov;104(11):3653-3680. https://doi.org/10.1002/jps.24610.

Stoica AE, Chircov C, Grumezescu AM. Nanomaterials for Wound Dressings: An Up-to-Date Overview. Molecules. 2020 Jun 10;25(11):2699. https://doi.org/10.3390/molecules25112699.

Derakhshandeh H, Kashaf SS, Aghabaglou F, Ghanavati IO, Tamayol A. Smart Bandages: The Future of Wound Care. Trends Biotechnol. 2018 Dec;36(12):1259-1274. https://doi.org/10.1016/j.tibtech.2018.07.007.

Kovalenko OM, Kovalenko AO, Osadcha OI. [Impact of the wound coverings on the wound in superficial burns of the skin]. Klin Khir. 2017;(2):28-30. Ukrainian.

Ananieva MM, Faustova MO, Loban GA, Avetikov DS, Basarab YaO, Ksonz VI. Microbiological aspects of chlorophyllipt extract used for prevention of candida postoperative complications. Euromediterranean Biomedical Journal. 2018;13(40):178-180.

Shi C, Wang C, Liu H, Li Q, Li R, Zhang Y, Liu Y, Shao Y, Wang J. Selection of Appropriate Wound Dressing for Various Wounds. Front Bioeng Biotechnol. 2020 Mar 19;8:182. https://doi.org/10.3389/fbioe.2020.00182.

Banes AJ, Compton DW, Bornhoeft J, Hicks H, Link GW, Bevin AG, Lawrence WT, Peterson HD. Biologic, biosynthetic, and synthetic dressings as temporary wound covers: a biochemical comparison. J Burn Care Rehabil. 1986 Mar- Apr;7(2):96-104. https://doi.org/10.1097/00004630- 198603000-00004.

Montheard J-P, Chatzopoulos M, Chappard D. 2-Hydroxyethyl Methacrylate (HEMA): Chemical Properties and Applications in Biomedical Fields. Journal of Macromolecular Science. 1992 Part C 32(1):1-34. https://doi.org/10.1080/15321799208018377.

Brahim S, Narinesingh D, Guiseppi-Elie A. Synthesis and hydration properties of pH-sensitive p(HEMA)- based hydrogels containing 3-(trimethoxysilyl) propyl methacrylate. Biomacromolecules. 2003 May-Jun;4(3):497-503. https://doi.org/10.1021/bm020080u.

Mokrý J, Karbanová J, Lukás J, Palecková V, Dvoránková B. Biocompatibility of HEMA copolymers designed for treatment of CNS diseases with polymer-encapsulated cells. Biotechnol Prog. 2000 Sep-Oct;16(5):897-904. https://doi.org/10.1021/ bp000113m.

Robb EC, Fitz DG, Nathan P. Delivery of the topical antimicrobial agents silver sulfadiazine, gentamicin and nystatin to infected burn wounds in rats from preloaded synthetic dressings. Trans Am Soc Artif Intern Organs. 1980;26:533-6.

Husain MT, Akhtar M, Akhtar N. Report on evaluation of hydron film as burn wound dressing. Burns Incl Therm Inj. 1983 May;9(5):330-4. https://doi.org/10.1016/0305-4179(83)90079-7.

Bulyga LO, Butko YaO. [New approaches to development of local wound healing]. Integrative Anthropology. 2017;29(1):37-40. Ukrainian.

Kukolevska OS, Gerashchenko II, Borysenko MV, Pakhlov EM, Machovsky M, Yushchenko TI. Synthesis and Examination of Nanocomposites Based on Poly(2-hydroxyethyl methacrylate) for Medicinal Use. Nanoscale Res Lett. 2017 Dec;12(1):133. https://doi.org/10.1186/s11671-017-1881-7.

Panasenko OI, Donchenko NV, Hotsulya AS, Buryak VP, Caitlin IN, Yurchenko IA, Postol NA. [UV spectrophotometry of the Metronidazole]. Problems of pharmacy. 2013;80(5);92-94. Ukrainian.

Davtyan LL, Voronkina AS, Chubenko ОV, Trohimchuk VV. [Development of methods of quality control for stomatologic films containing chlorhexidine, metronidazole and glucosamine]. Farm Zn. 2016;3-4:92-99. Ukrainian. URL: http://nbuv.gov.ua/UJRN/pharmazh_2016_3-4_16.

Zhebentyaev AI. [Spectrophotometric determination of decamethoxin with eosin]. Izv Vyssh Uchebn Zaved Khim Khim Tekhnol. 1984;27(4):412-414. Russian.

Nazarchuk OA, Chereshniuk IL, Nazarchuk HH. The research of antimicrobial efficacy of antiseptics decamethoxin, miramistin and their effect on nuclear DNA fragmentation and epithelial cell cycle. Wiad Lek. 2019;72(3):374-380. PMID: 31050983.

Plaut BS, Davies DJ, Meakin BJ, Richardson NE. The mechanism of interaction between chlorhexidine digluconate and poly(2-hydroxyethyl methacrylate). J Pharm Pharmacol. 1981 Feb;33(2):82-8. https://doi.org/10.1111/j.2042-7158.1981.tb13716.x.

Kukolevska OS, Siora IV, Chornopyshchuk RM, Gerashchenko II. [Medical and biological research of nanocomposite material «Polydens»]. Chemistry, Physics and Technology of Surface. 2016;7(2):225-235. Ukrainian. https://doi. org/10.15407/hftp07.02.225.

Кukolevska О, Gerashchenko I, Pakhlov E. [Nanocomposite materials with controlled release of bioactive substances]. NaUKMA Research Papers. 2016;183:60-64. Ukrainian.

Zheliba MD. et al. (2016) Assessment of the Antimicrobial Activity of Polymer Materials with Added Nanosilica Modified by Silver Compounds. In: Sontea V., Tiginyanu I. (eds) 3rd International Conference on Nanotechnologies and Biomedical Engineering. IFMBE Proceedings, vol 55. Springer, Singapore. https://doi.org/10.1007/978-981-287- 736-9_64

Farhatnia Y, Tan A, Motiwala A, Cousins BG, Seifalian AM. Evolution of covered stents in the contemporary era: clinical application, materials and manufacturing strategies using nanotechnology. Biotechnol Adv. 2013 Sep-Oct;31(5):524-42. https://doi.org/10.1016/j.biotechadv.2012.12.010. Epub 2013 Jan 7. PMID: 23305892.

Lee CH, Hsieh MJ, Liu SC, Chen JK, Liu SJ, Hsieh IC, Wen MS, Hung KC. Novel bifurcation stents coated with bioabsorbable nanofibers with extended and controlled release of rosuvastatin and paclitaxel. Mater Sci Eng C Mater Biol Appl. 2018 Jul 1;88:61-69. https://doi.org/10.1016/j.msec.2018.02.027. Epub 2018 Mar 2. PMID: 29636139.

Peppas NA, Lustig SR. The role of cross- links, entanglements, and relaxations of the macromolecular carrier in the diffusional release of biologically active materials. Conceptual and scaling relationships. Ann N Y Acad Sci. 1985;446:26-41. https://doi.org/10.1111/j.1749-6632.1985.tb18388.x. PMID: 3860156.