Protective Role of Clinacanthus nutans Against Liver Damage in Streptozotocin-induced Diabetic Rats

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Published: Nov 30, 2024
DOI: https://doi.org/10.47836/mjmhs20.6.4
Keywords:
Clinacanthus nutans, Diabetes, Liver damage, Oxidative stress, Transaminases

Main Article Content

Nurlaili Susanti
Department of Biomedicine, Faculty of Medicine and Health Science, Maulana Malik Ibrahim State Islamic University, Malang
Putri Wulan Akbar
Department of Biomedicine, Faculty of Medicine and Health Science, Maulana Malik Ibrahim State Islamic University, Malang
Aulia Sri Nastiti Suwondo
Department of Biomedicine, Faculty of Medicine and Health Science, Maulana Malik Ibrahim State Islamic University, Malang
Firdaus Silahudin
Department of Biomedicine, Faculty of Medicine and Health Science, Maulana Malik Ibrahim State Islamic University, Malang
Mahasya Khafiralia
Department of Biomedicine, Faculty of Medicine and Health Science, Maulana Malik Ibrahim State Islamic University, Malang
Muhamad Daffa Abhista Reviansyah
Department of Biomedicine, Faculty of Medicine and Health Science, Maulana Malik Ibrahim State Islamic University, Malang
Nur Alqurratu A’yuni Syukri
Department of Biomedicine, Faculty of Medicine and Health Science, Maulana Malik Ibrahim State Islamic University, Malang
Rasyidu Fabian Mashuri
Department of Biomedicine, Faculty of Medicine and Health Science, Maulana Malik Ibrahim State Islamic University, Malang

Abstract

Introduction: Diabetes mellitus (DM) triggers oxidative stress and damages many organs, including the liver. The development of herbal medicine is an important approach to preventing liver damage due to DM. This study investigated the effects of ethanolic extract of Clinacanthus nutans (EECN) on the liver anti-oxidant status and tissue morphology of diabetic rats. Materials and methods: Five groups of Male Wistar rats were comprised of healthy control rats (Group 1); untreated diabetic control rats (Group 2); diabetic rats given EECN 100 mg/kg BW (Group 3); diabetic rats given EECN 200 mg/kg BW (Group 4); and diabetic rats given EECN 400 mg/kg BW (Group 5). After 28 days of treatment, the rats were terminated. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations were measured from blood serum. Malondialdehyde (MDA) and Superoxide dismutase (SOD) levels were measured from liver tissue homogenates. Liver tissue was stained with Hematoxylin-Eosin and observed with a light microscope. Results: Serum ALT and AST, as well as liver MDA levels, decreased, while liver SOD levels increased in the EECN group compared to untreated diabetic rats. In addition, lower degenerative and necrotic changes in hepatocytes were also observed in the EECN group. Conclusion: EECN protects against liver tissue damage, as evidenced by reduced transaminases, oxidative stress, and degenerative hepatocytes.

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Susanti, N., Akbar, P. W., Suwondo, A. S. N., Silahudin, F., Khafiralia, M., Reviansyah, M. D. A., Syukri, N. A. A., & Mashuri, R. F. (2024). Protective Role of Clinacanthus nutans Against Liver Damage in Streptozotocin-induced Diabetic Rats. Malaysian Journal of Medicine and Health Sciences, 20(6), 19–24. https://doi.org/10.47836/mjmhs20.6.4
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Vol. 20 No. 6 (2024)
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Original Articles
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