The Effect of Lipopolysaccharide Exposure During Pregnancy on Hepcidin Expression in Female Mice: Involvement of Interleukin-6 and Activin B, Independent of Transforming Growth Factor-β Receptor

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Published: Nov 30, 2024
DOI: https://doi.org/10.47836/mjmhs20.6.7
Keywords:
Activin B, Estradiol, Hepcidin, Inflammation, Interleukin-6, Pregnancy

Main Article Content

Aryati
Department of Clinical Pathology, School of Medicine, Universitas Airlangga, 60286 Surabaya, Indonesia
Gilang Nugraha
Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, 60132 Surabaya, Indonesia; Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, 60237 Surabaya, Indonesia
Widjiati Nugraha
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, 60115 Surabaya, Indonesia
Citrawati Dyah Kencono Wungu
Department of Biochemistry, Faculty of Medicine, Universitas Airlangga, 60132 Surabaya, Indonesia
Harianto Notopuro
Department of Biochemistry, Faculty of Medicine, Universitas Airlangga, 60132 Surabaya, Indonesia
Win Darmanto
Department of Biology, Faculty of Science and Technology, Universitas Airlangga, 60115 Surabaya, Indonesia
Agus Sulistyono
Maternal–Fetal Medicine Division, Obstetrics & Gynecology Department, Dr. Soetomo Academic General Hospital, Universitas Airlangga, 60286 Surabaya, Indonesia
Hari Basuki Notobroto
Department of Biostatistics & Population, Faculty of Public Health, Universitas Airlangga, 60115 Surabaya, Indonesia
Purwo Sri Rejeki
Department of Physiology, Faculty of Medicine, Universitas Airlangga, 60132 Surabaya, Indonesia

Abstract

Introduction: The expression of hepcidin in hepatocytes is induced by inflammation, primarily mediated by interleukin 6 (IL-6) and activin B. In contrast, hepcidin levels are suppressed during pregnancy via the half-site of the estrogen-responsive element (ERE) on the hepcidin gene promoter. These opposing mechanisms regulate hepcidin in pregnant women with inflammation. However, several studies report no change in hepcidin levels in the blood of pregnant women experiencing inflammation. Therefore, this study investigated the expression of hepcidin in pregnant mice with induced inflammation using LPS injection. Materials and methods: This study involved 26 pregnant mice. The treatment group was injected intraperitoneally with serotype O111:B4 (Sigma-Aldrich, Merck, Singapore), while the control group received Phosphate Buffered Saline (PBS). Serum levels of IL-6, activin B, estradiol, and hepcidin were measured using ELISA. The liver tissues were examined via immunohistochemistry to measure the Transforming Growth Factor-β (TGF-β) receptor. Data were analyzed using an independent t-test. Results: Our results demonstrated that pregnant mice with inflammation had significantly increased IL-6 (P = 0.000) and decreased activin B (P = 0.032) levels, but there were no significant differences in estradiol (P = 0.624), hepcidin (P = 0.607), and TGF-β receptor levels (P = 0.662). Conclusion: Our study showed that inflammation during pregnancy does not impact hepcidin levels. Additionally, we observed a decrease in activin B levels in pregnant mice with inflammation. Conversely, high levels of estradiol during pregnancy may contribute to the suppression of hepcidin synthesis.

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How to Cite
Aryati, Nugraha, G., Nugraha, W., Wungu, C. D. K., Notopuro, H., Darmanto, W., Sulistyono, A., Notobroto, H. B., & Rejeki, P. S. (2024). The Effect of Lipopolysaccharide Exposure During Pregnancy on Hepcidin Expression in Female Mice: Involvement of Interleukin-6 and Activin B, Independent of Transforming Growth Factor-β Receptor. Malaysian Journal of Medicine and Health Sciences, 20(6), 38–43. https://doi.org/10.47836/mjmhs20.6.7
Issue
Vol. 20 No. 6 (2024)
Section
Original Articles
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